GJB2 and KID syndrome: To examine the effects of the KID syndrome-associated Gjb2 mutations Cx26-G12R and -G45E on the intracellular localization of Cx26, HeLa (human cervical carcinoma) cells lacking endogenous gap junctions were transiently transfected with pIRES2-AcGFP1 Cx26-WT (wild-type), -G12R or -G45E-FLAG constructs (pIRES2-AcGFP1 Gjb2 WT, c.34 G > C or c.134 G > A-FLAG constructs).