PTEN and neoplasm: Next, immunofluorescence and immunohistochemistry assays revealed that siRP11-79H23.3 group cells revealed much lower PTEN expression as well as a stronger positive signal of p-PI3K and p-Akt expression in tumor tissue of nude mice, whereas higher PTEN expression as well as weaker brown immunostain and fluorescence intensity of p-PI3K and p-Akt were seen in tumor xenograft tissue of RP11-79H23.3 cells group (Figure 5D–G).