MDA-MB-231 cells were treated with this inhibitor for 24 h and subjected to the ALDEFLUOR assay to investigate the effect of 3-O-trans-p-coumaroyltormentic acid on the proportion of ALDH-positive breast cancer cells; the results indicated that this compound decreased the proportion of ALDH-positive breast cancer cells from 1.0 to 0.4% (Figure 4B). The gene discussed is LDHA; the disease is breast cancer.