In the present study, we compared the impact of yeast-derived recombinant Avns, YAvnI and II, and major natural Avns, Avn-A and Avn-C, on colon cancer cells, showing that YAvns were as effective as natural Avns in inhibiting tumor cell growth and survival, but were more effective in inhibiting EMT and reducing tumor cell migration through transcriptional regulation of E-cadherin, suggesting enhanced functional and nutraceutical properties that might be advantageous with respect to colon cancer management and metastasis prevention. Here, CDH1 is linked to malignant colon neoplasm.