In the current study, in order to continue with our efforts to screen mushrooms that manifest anticancer potential against lung cancer and identify compounds that contribute to the activity [19,20,21], we evaluated the biological activity of an EtOH extract of the sclerotia of P. cocos in four human lung adenocarcinoma cell lines, A549, H1264, H1299, and Calu-6, accompanying different p53 status. Here, TP53 is linked to lung cancer.