METTL17 and neoplasm: Anticancer therapies inhibit tumor growth by affecting proliferation, and to determine whether METTL17 silencing altered cell proliferation, we performed trypan blue staining of HCC827 cells expressing either negative-control sgRNA (Neg 1) or stable silencing of METTL17 that were exposed to either control medium or medium containing 20 mM 2DG over 48 hours.