TNF and peritonitis: Another author described the in vivo anti-inflammatory effect by lupeol acetate; it has a significative anti-inflammatory activity by regulating TNF-α and IL-2 and involves the opioids system; in this study the author uses morphine as control drug; the assays were formalin test (2nd phase), carrageenan-induced paw edema, dextran-induced paw edema, and peritonitis induced by carrageenan [19].