Molecular analysis of DCIS.COM-derived myoepithelial-specific (integrin β6+) and luminal specific (MUC1+) cells led to the discovery that a complex interaction between epithelial, myoepithelial and stromal signaling pathways including TGFβ, hedgehog, cell adhesion and p63 were required for the loss of myoepithelial cells in DCIS and progression to invasion [81]. This evidence concerns the gene TGFB1 and ductal breast carcinoma in situ.