For this analysis, we developed a 3D collagen matrix and microfluidics-based cell cultures to quantify the plasticity of the migratory capacity of metastatic non-small cell lung cancer (NSCLC) cells (H1299 cells) and evaluate how cell behaviors depend on the collagen concentration and the presence of TGF-β. Here, TGFB1 is linked to non-small cell lung carcinoma.