The predicted network provides a global aspect for PPI of the key protein coding genes that could be involved in ESCC e.g STAT3 (Signal Transducer and Activator of Transcription 3), which is used as the bait protein in PEPPER PPI analysis clearly shows direct interactions between FGFR2 (Fibroblast growth factor receptors) and CCND1 (Cyclin D1). Here, STAT3 is linked to esophageal squamous cell carcinoma.