The abilities of scAb-EGFR-PEG-g-PEI-SPION/PBOV1-pDNA (for TO group) to effectively upregulate, whereas scAb-EGFR-PEG-g-PEI-SPION/PBOV1-psiRNA (for TS1 and TS2 groups) to effectively downregulate the PBOV1 protein expression were validated in Hep3B, SMMC-7721, and BEL7402 HCC cell lines. The gene discussed is EGFR; the disease is hepatocellular carcinoma.