Delivery of PBOV1 plasmid (PBOV1-pDNA) and PBOV1-siRNA plasmid (PBOV1-psiRNA) into HCC cells could up and downregulate the PBOV1 gene expression, respectively, upon which we hoped to understand whether and how PBOV1 expression levels affected the growth and metastasis of HCC. The gene discussed is PBOV1; the disease is hepatocellular carcinoma.