The role of Trx1 in preventing cardiac hypertrophy (1) Dominant negative (ND) mutant (C32S, C35S) of Trx1 diminished the activity of Trx1 (2) Elevation of oxidative stress (3) Cardiac hypertrophy Enhance Ras-Raf-1-ERK pathway. The gene discussed is TXN; the disease is cardiac hypertrophy.