ACTA1 and colonic neoplasm: To confirm the colocalization of IntegriSense probe with ECM proteins, tissues were analysed for the expression of α-smooth muscle actin (α-SMA) and fibrin: as expected, we observed several spots of colocalization between IntegriSense and both α-SMA and fibrin in small intestine and colon tumors (Figure 7C and 7D, respectively).