Therefore, in this work, since the Notch-1 and OPN molecules are related to the maintenance of angiogenesis and GSLCs, we analyze the distribution of Notch-1 and OPN immunopositivity in relation to nestin and CD133 and the proangiogenic factor VEGF in early to advanced stages of ENU-gliomas. The gene discussed is NOTCH1; the disease is central nervous system cancer.