To further support the notion that NETs drive the development of atherosclerosis and that PAD4 KO mice have decreased atherosclerosis due to hampered NET formation, WT mice and PAD4 KO were administered DNase I. Treatment with DNase I did not modify rates of weight gain (or spleen weight/body weight ratios) (Figures S4A,B in Supplementary Material). The gene discussed is PADI4; the disease is atherosclerosis.