Furthermore, by using mimic to up-regulate and inhibitor to down-regulate microRNA375 in EGC, we demonstrated that up-regulated expression of microRNA375 can induce EGC apoptosis, down-regulated expression of microRNA375 can prevent the hyperglycemia-induced EGC apoptosis, and these effects of microRNA375 were partly through targeting Pdk1 and suppressing PI3K/Akt pathway. This evidence concerns the gene PDK1 and Hyperglycemia.