RT-qPCR of select cancer-related genes in both shRING1B T47D and MDA-MB-231 cells confirmed the RNA-seq results, and further suggested that fatty acid metabolism (represented by CD36 and HMGCS2) may play a major role in the tumorigenesis of ER+ breast cancer (Fig. 4c). This evidence concerns the gene HMGCS2 and breast carcinoma.