This approach has been used to study other variants implicated in hearing loss, including for MYO15A, STRC, TJP2 and USH2A [18, 19], The minigene experiment described here was designed to evaluate the expression pattern of c.9083+6T>A and revealed a differential splicing pattern between the wild type and the mutant alleles. Here, MYO15A is linked to hearing loss disorder.