ANXA5 and melanoma: To investigate whether the antiproliferative action of the combined administration of SIM and DMXAA might be associated with the induction of apoptosis/necrosis in B16.F10 murine melanoma cells co-cultured with TAMs, we performed double labeling: for apoptosis with Annexin V-FITC and for necrosis with PI, as previously described [23].