As indicated in Figures 3A,B, after exposure to lycorine, CRC cells showed an up-regulation of Bax and a down-regulation of Bcl-2, which led to a dose-dependent increase of the ratio of Bax/Bcl-2, and an increased cell proportion of depolarized mitochondria after being treated with lycorine (Figure 3C). The gene discussed is BAX; the disease is colorectal carcinoma.