Consistently, western blotting assay in eight representative AML BM blast samples and seven AML cell lines across M2 to M6 subtypes indicated that among the common AML subtypes, a sharp decrease in JMJD3 protein level most likely occurred in M2 and M3 subtypes, and that among M4 or M5 subtypes, a moderate reduction was not consistently detected (Fig. 1d, e). This evidence concerns the gene KDM6B and acute myeloid leukemia.