In line with the above observations that C/EBPβ partnered with JMJD3, upon the forced JMJD3 overexpression, to regulate the myeloid differentiation program of AML cells, western blotting assay showed that ATRA induced a significant induction of JMJD3 in parallel to the induction of C/EBPβ in HL-60 cells, and that JMJD3 deficiency compromised the ATRA-induced C/EBPβ upregulation and myeloid differentiation (Fig. 7a, b). This evidence concerns the gene KDM6B and acute myeloid leukemia.