In all animals, blood monocytosis occurred 2–4 dpi (Fig. 2c), a finding that is consistent with studies in ZIKV infected patients and other NHP models9,27,28, corresponding to increases in all monocytes subsets within the first 4 days of infection (Supplementary Fig. 4) and declines in CD86 expression in classical blood monocytes occurred throughout ZIKV infection starting at 1 dpi and extending through 25 dpi (Supplementary Fig. 2). Here, CD86 is linked to Zika virus infectious disease.