Furthermore, we found that GCNT2 gene expression levels directly correlated with the presence of cell surface I-branched glycans (Supplementary Fig. 2a, b) and culturing GCNT2/I-branched glycan expressing melanoma cells in the presence of complex N-glycan inhibitor, kifunensine, and glycolipid inhibitor, D,l-threo-1-phenyl-2-hexadecanoylamino-3-pyrrolidino-1-propanol-HCl (PPPP), confirmed that I-branched glycans are present on N-linked glycoproteins as well as on glycolipids in melanoma cells (Supplementary Fig. 2c–i). This evidence concerns the gene GCNT2 and melanoma.