IL6R and ductal breast carcinoma in situ: A significant increase in the mRNA and protein expression levels of IL-6 was observed in hPreAd in co-culture and after treatment with conditioned media compared to those in the single culture, and the mRNA and protein expression levels of IL-6R were not altered in DCIS.com in co-culture or after treatment with conditioned media (Fig. 3c, d, and e).