A significant increase in the mRNA and protein expression levels of IL-6 was observed in hPreAd in co-culture and after treatment with conditioned media compared to those in the single culture, and the mRNA and protein expression levels of IL-6R were not altered in DCIS.com in co-culture or after treatment with conditioned media (Fig. 3c, d, and e). The gene discussed is IL6; the disease is ductal breast carcinoma in situ.