First of all, four NSCLC cell lines including A549 and CL1–5 (WT EGFR), HCC827 (del E746-A750), and H1975 (L858R/T790 M) were treated with various concentrations (0~ 80 μM) of API for 24 or 48 h to determine the cytotoxic effects of API. The gene discussed is EGFR; the disease is non-small cell lung carcinoma.