GSK (10 nM) was used as a selective TRPV4 agonist, which showed a rapid and reversible calcium influx of 496 ± 366 nM in endometriosis-derived hESC and 484 ± 277 nM in control hESC, in, respectively, 37 ± 18% (total of 305 cells) and 28 + 16% of the cells (total of 241 cells) (Figure 3B,E,F and Figure S3B). Here, TRPV4 is linked to endometriosis.