In addition to our histologic findings in human PD tissues, we conducted dual-labeling immunocytochemistry experiments using the differentiated LUHMES cell model of human DA neurons.32 We observed induction of NLRP3 in neurons following treatment with inflammasome-activating agent Nigericin following lipopolysaccharide (LPS) priming in Tuj1-positive neuronal cells. Here, NLRP3 is linked to Parkinson disease.