We analyzed The Cancer Genome Atlas (TCGA) database to look for mutually exclusive patterns between NF1 nonsense mutations and CAPN1. As can be seen in Figure 1C, the CAPN1 (11q13.1) locus was amplified in 5% of human melanoma cases, and was consistently retained in a mutually exclusive fashion in NF1-deficient melanomas (P = 0.024, the FDR corrected P-value was calculated using WEXT) [35], further suggesting that they are found in the same functional pathway. The gene discussed is NF1; the disease is melanoma.