Here, combined inhibition of MEK and CAPN1 by simultaneous treatment with Trametinib and Calpain Inhibitor I achieved greater efficacy than Trametinib alone both in wild-type NF1 melanoma cells (A375 and 74T) and in melanoma cells that harbor one NF1 mutant allele and another wild-type one (108T and 76T) (Figure 5). The gene discussed is CAPN1; the disease is melanoma.