In order to test the specificity of the CAPN1 inhibition effect in stabilizing NF1 protein levels, decreasing RAS/AKT signaling pathways and reducing cell proliferation in NF1-wild type melanoma cells, we tested the effect of CAPN1 inhibition on an NF1-null melanoma cell line, CO84, which was derived from a melanoma patient, that harbored the p.R2517*NF1 truncation mutation. Here, AKT1 is linked to melanoma.