To test the impact of pregravid obesity on the functional responses of innate immune cells, we measured cytokine, chemokine, and growth factor production from PBMC following overnight stimulation with TLR2 (Pam3CSK4), 3 (PolyI:C), 4 (LPS), 6 (FSL-1), 7 (Imiquimod), and 9 (ODN2216) ligands. This evidence concerns the gene TLR2 and obesity due to melanocortin 4 receptor deficiency.