Thus, the 257 genes were subjected to pathway enrichment analysis using GeneAnalytics [34], and the results revealed that Hippo pathway, TNF signaling, Wnt-mediated β-catenin signaling, TGF-beta receptor signaling pathway, TP53 network, and regulation of activated PAK-2p34 by proteasome mediated degradation pathway were most affected dysregulating pathways in ESCC (Additional file 1: Table S5). The gene discussed is TNF; the disease is esophageal squamous cell carcinoma.