Based on the dysregulation of the immune system of autism, and the anti-inflammatory characteristics of hydrogen, the present study aimed to investigate the potential effects of HRW on autistic-like behaviors using a maternal valproic acid (VPA)-exposed-mice model, and explore the associated mechanism focusing on the peripheral serum levels of interleukin 6 (IL-6) and tumor necrosis factor-α (TNF-α) assessed by Enzyme-Linked Immune-Sorbent Assay (ELISA) analysis. Here, IL6 is linked to autism.