Second, qRT-PCR analysis also revealed that C14orf166 mRNA was overexpressed in most cases of NSCLC, which was positively associated with the IHC staining score, indicating that C14orf166 was up-regulated in NSCLC both transcriptionally and translationally, which was in accordance with the function of C14orf166. Here, RTRAF is linked to non-small cell lung carcinoma.