Because the levels of three protein-coding NPM1 transcripts were increased in leukemia compared to HV and RNA-seq data analysis suggested the decrease of NPM1 transcript level after therapy, we used ddPCR to test how the levels of three protein-coding NPM1 transcripts changed at three time points: at the time of first diagnosis (AML-T0), after therapy (AML-T1) and at relapse (AML-T2). The gene discussed is NPM1; the disease is acute myeloid leukemia.