PDK1 and infection: As shown in Fig 4N and 4O, only infection with the wild-type EPEC E2348/69 or the mutant strain ΔnleBE+pNleB resulted in significant induction of SLC2A1 (GLUT1) and PDK1, but in cells that were infected with the mutant strain ΔescN+pNleB, the control strain ΔnleBE+vector, or the mutant strain ΔnleBE+pNleB-DXD, there was no significant effect on the induction of SLC2A1 (GLUT1) and PDK1 (Fig 4N and 4O).