EPAS1 and infection: In RCC4 cells (which contain both HIF-1α and HIF-2α) [30], ΔnleBE+pNleB infection caused higher cellular uptake of 2-NBDG (a fluorescence glucose analog) as quantified by flow cytometry (FACS) compared with cells infected with the control (nleBE+vector or ΔnleBE+pNleB-DXD strains) (p = 0.0114) (Fig 7A).