The combination of SIM andROM caused simultaneous downregulation of BCL11aand HDAC1 but significantly increased HbF expression.Similarly, Elizabeth et al. showed that combination ofSIM and t-butylhydroquinone increases Gamma-globinexpression 3.2-fold higher than the control group.Currently, SIM is using reduction of cholesterol andprevention of cardiovascular diseases. This evidence concerns the gene HDAC1 and cardiovascular disorder.