For instance, Pitter et al. (2011) demonstrated that the vertical inhibition of Akt/mTOR pathway by the use of perifosine (Akt inhibitor) and temsirolimus (mTOR inhibitor) decreased tumor proliferation and induced apoptosis both in vitro and in vivo models of GBM, thus suggesting that the combination acts in synergy to inhibit the Akt/mTOR axis. This evidence concerns the gene MTOR and glioblastoma.