To investigate the effect of the circ-ZEB1.33-miR-200a-3p-CDK6 on the cell biology of HCC cell, we generated a serial of cell lines based on this regulatory axis including circ-ZEB1.33 overexpression (Huh7-circ-ZEB1.33), circ-ZEB1.33 knockdown (Huh7-shcirc-ZEB1.33), and circ-ZEB1.33 overexpression plus CDK6 knockdown (Huh7-circ-ZEB1.33+shCDK6). Here, CDK6 is linked to hepatocellular carcinoma.