The current study suggests that the determination of ESR1, PGR, ERBB2, and MKi67 RNA levels by RT-qPCR on the GeneXpert automated diagnostic platform is not only feasible, but also generates results from FFPE tumor sections that are highly concordant with high quality central laboratory measurements of ER, PR, and HER2 using standardized IHC and FISH assays. This evidence concerns the gene PGR and neoplasm.