To test the cellular impact of reduced RARγ expression levels we generated clones with stable knocked-down of RARγ in non-malignant prostate epithelial cells (RWPE-1) and LNCaP PCa cells using two separate RARγ targeting short hairpin RNA (shRNA) constructs (Fig. 2a–d). The gene discussed is RARG; the disease is posterior cortical atrophy.