ZIC3 and right atrial isomerism: We used Sanger sequencing to confirm the variants observed in the family pedigrees and identified a novel hemizygous mutation (c.890G > T, p.C297F) in the X-linked ZIC3 gene in a 13-month-old male patient with asplenia syndrome (also known as right atrial isomerism), right stomach, left liver, TGA and DORV (Fig. 1a and b).