In detail, at day 14 and 21 post-infection, two dimensional (2D) plotting of PCoA-1 versus PCoA-2 failed to show a pattern of clustering between eif2ak4+/+ + PBS and eif2ak4+/+ + LF82 groups (Fig. 3B,F), indicating that colonization by AIEC may not alter microbiota composition in wild type host. The gene discussed is EIF2AK4; the disease is infection.