Keeping in mind that both fibroblast‐specific protein‐1 (Fsp1) and α‐smooth muscle actin (αSMA) were reported as mesenchymal cell markers associated with EMT in pancreatic cancer (Kalluri & Weinberg, 2009; Wang et al, 2009; Rhim et al, 2012; Zheng et al, 2015; Aiello et al, 2016; Li et al, 2016), we bred the KPF strain with αSMA‐Cre or Fsp1‐Cre transgenic mice combined with a novel dual‐fluorescence‐switchable reporter, R26Dual (Rosa26‐CAG‐loxP‐frt‐Stop‐frt‐FireflyLuc‐EGFP‐loxP‐RenillaLuc‐tdTomato). This evidence concerns the gene S100A4 and pancreatic neoplasm.