Both Rnf43 and Znrf3 were expressed at significantly lower levels in epithelium from VillinCreAhrfl/fl and VillinCreR26LSL-Cyp1a1 mice, while WNT target genes such as Axin2, cMyc, and Ephb2 were expressed at higher levels in steady-state conditions (Figure S1A) and following AOM/DSS administration (Figure 4F). Here, CYP1A1 is linked to infectious otitis media.