To further validate whether KRAL inhibits the Nrf2 pathway by regulating Keap1 expression in a dose-dependent manner, parental and resistant HCC cells were co-transfected with HO-1-ARE luciferase plasmid or control vector (10 ng) and shR-KRAL-1/2 plasmids or pLV-KRAL plasmids, combined either miR-141 inhibitor or miR-141 mimics, respectively. Here, KEAP1 is linked to hepatocellular carcinoma.