Subsequently, to explore whether KRAL regulated Keap1 expression through the regulation of Keap1–3’UTR, the pmirGLO-Keap1–3’UTR plasmid was co-transfected with the pLV-KRAL1/2 plasmids, shR-KRAL-1/2 plasmids, and either miR-141 mimics or miR-141 inhibitor in resistant HCC cells and their parental strains, respectively. Here, KEAP1 is linked to hepatocellular carcinoma.