In contrast, silencing KRAL could decrease the Keap1 levels and enhance the expression of genes downstream of the Nrf2 pathway, thus promoting 5-FU resistance in HCC cells, while ectopically expressing Keap1 or silencing miR-141 partially abolished KRAL knockdown-mediated changes (Fig. 9b). This evidence concerns the gene NFE2L2 and hepatocellular carcinoma.