ATG5 and acute myeloid leukemia: Therefore, autophagy was evaluated in the AML cells by immunoblotting analysis of the Atg5‐Atg12 complex, LC3 processing (I and II) and LC3 puncta.44 KG‐1 cells presented the highest Atg5‐Atg12 complex protein levels (Figure 3A) associated with the highest autophagy flux, as reflected by the LC3 processing (Figure 3B).