CRISPR/Cas9 deletion of a 57 bp region containing rs56069439 reduced ANKLE1, but not ABHD8 or BABAM1 expression; overexpression of ABHD8 reduced cell migration and invasion and caused expression changes in cancer-related pathways, overexpression of ANKLE1 caused expression changes in cancer-associated and cell growth/proliferation pathways. This evidence concerns the gene ANKLE1 and cancer.