HBV-related HCC specimens (Fig. 5C), HBx transgenic mouse model and (Fig. 5D) and miR-224 overexpression enhanced and GNMT overexpression reduced tumor growth in vivo, which is consistent with the in vitro data (Fig. 4 and Supplementary Fig. S3), In addition, AAV-GNMT reduced CCl4-induced miR-224 expression and fibrosis formation in mouse liver (Fig. 6B,C,F). This evidence concerns the gene GNMT and neoplasm.