In order to determine the functions of KIFC1 in HCC, we performed gain- and loss-of-function experiments by overexpressing KIFC1 in SMMC7221 and Huh7 (low metastatic potential HCC cell lines) and by silencing KIFC1 in HCCLM3 and SK-Hep-1 (high metastatic potential), respectively, by lentiviral infection. Here, KIFC1 is linked to hepatocellular carcinoma.