In this study, we show that the pan-DUB inhibitor PR-619 reduces expression of mesenchymal markers, deposition of ECM proteins, expression of MMP2 and MMP9, the degree of apoptosis, macrophage infiltration, and the TGF-β1 mRNA level, and attenuates renal fibrosis. Here, TGFB1 is linked to renal fibrosis.