TNF and neoplasm: At 3 w p.i. 4T1 + RAW264.7 primary tumor lysates showed a significant increase in the M1-related cytokines IL-1β, monocyte chemoattractant protein (MCP)-1, macrophage inflammatory protein (MIP)-2 and TNF-α compared to the 4T1 primary tumors, whereas the M2-related cytokines BAFF, G-CSF, IL-4 and TGF-β1 showed significantly lower levels in the 4T1 + RAW264.7 versus the 4T1 primary tumors (Fig. 5a and b).